Table 1.

Expression of lncIHAT in different subtypes of breast cancer cell lines.

SubtypeCell lineΔCt20% O2ΔCt1% O2Hypoxia induction (2−Δ(ΔCt1% O2Ct20% O2))Basal level under 20% O2 relative to ZR-75-1 cells (2−Δ(ΔCt(cell line)-ΔCt(ZR-75-1)))
LuminalZR-75-124.12 ± 0.1721.26 ± 0.397.45 ± 2.13b1.00 ± 0.12
T47D21.09 ± 0.5417.55 ± 0.3011.77 ± 2.33b8.54 ± 2.84
MCF-720.49 ± 1.1616.50 ± 0.2116.00 ± 2.25c14.80 ± 8.58
HER2+BT47423.23 ± 0.2319.38 ± 0.0914.48 ± 0.93d1.87 ± 0.31
HCC195420.12 ± 0.4518.04 ± 0.144.23 ± 0.41c16.53 ± 4.63
TNBCBT-2017.64 ± 0.7013.88 ± 0.6614.57 ± 7.19a96.94 ± 47.14
MDA-MB-23117.59 ± 0.3915.33 ± 0.274.86 ± 0.96b94.65 ± 26.18
MDA-MB-46817.23 ± 0.3814.55 ± 0.266.49 ± 1.10b121.22 ± 30.88
SUM15915.56 ± 0.2114.57 ± 0.112.00 ± 0.15b379.71 ± 54.10
  • Note: A panel of breast cancer cell lines were exposed to 20% or 1% O2 for 24 hours. lncIHAT was quantified by qRT-PCR and normalized to an internal control 18S rRNA (mean ± SD, n = 3).

  • aP < 0.05.

  • bP < 0.01.

  • cP < 0.001.

  • dP < 0.0001 versus 20% O2 by Student t test.