Table 1.

Cancer stem cells are enriched by TP53 mutation

Number of spheroids
Number of cellsTP53WTTP53E3TP53E10
1,000165221330
50080139113
250305145
125111716
Spheroid-forming frequency (95% confidence interval)1/4,258 (1/4,785–1/3,788)1/2,728 (1/3,004–1/2,478)1/2,212 (1/2,417–1/2,025)
P (vs. TP53WT)4.18-E092.07E-19
P (vs. TP53E10)2.07E-191.46E-03
  • NOTE: LS174T cells were cultured at various densities (1,000, 500, 250, and 125 cells/well). The numbers of spheroids were counted after 7 days. The 95% confidence intervals for the spheroid-forming frequencies were calculated using ELDA software application. n = 5.