Table 1.

Selected selumetinib-regulated phosphopeptides

A427PhosphositeSequenceLog2FC
MEK–ERK pathway
 ERK1pT202/pY204IADPEHDHTGFLT(ph)EY(ph)VATR−1.61
 MEK1pS222LCDFGVSGQLIDSMANS(ph)FVGTR4.46
 BRAFpS605SRWS(ph)GSHQFEQLSGSILWMAPEVIR1.76
 CRAFpS296/pS301SHSESASPSALSSS(ph)PNNLS(ph)PTGWSQPK2.83
 RSK2pT365T(ph)PKDSPGIPPSANAHQLFR−2.20
 RSK1pS221KAYS(ph)FCGTVEYMAPEVVNR2.72
RTK
 EPHA2pY772VLEDDPEATY(ph)TTSGGKIPIR−0.42
 EPHA7pY791VIEDDPEAVY(ph)TTTGGKIPVR0.92
Signaling kinase
 AMPKA1pS486SIDDEITEAKS(ph)GTATPQR1.44
 PAK2pT169GTEAPAVVT(ph)EEEDDDEETAPPVIAPRPDHTK3.04
 MKK4pS90LKIS(ph)PEQHWDFTAEDLKDLGEIGR1.57
A549PhosphositeSequenceLog2FC
MEK–ERK pathway
 ERK1pT202/pY204IADPEHDHTGFLT(ph)EY(ph)VATR−3.09
 MEK1pS222LCDFGVSGQLIDSMANS(ph)FVGTR2.38
 BRAFpS605SRWS(ph)GSHQFEQLSGSILWMAPEVIR0.53
 CRAFpS296/pS301SHSESASPSALSSS(ph)PNNLS(ph)PTGWSQPK0.34
 RSK2pT365T(ph)PKDSPGIPPSANAHQLFR−2.40
 RSK1pS221KAYS(ph)FCGTVEYMAPEVVNR1.22
RTK
 METpT977/pS985VHT(ph)PHLDRLVS(ph)AR−1.20
 EGFRpS991/pS995MHLPS(ph)PTDS(ph)NFYR0.68
 EPHA2pY772VLEDDPEATY(ph)TTSGGKIPIR−1.62
Signaling kinase
 AMPKA1pS486SIDDEITEAKS(ph)GTATPQR1.34
 PAK2pT169GTEAPAVVT(ph)EEEDDDEETAPPVIAPRPDHTK1.04
 MKK3pT218MCDFGISGYLVDS(ph)VAKTMDAGCKPYMAPER−1.50
 MKK4pS90LKIS(ph)PEQHWDFTAEDLKDLGEIGR0.54
  • Abbreviation: Log2FC, log2 fold change.