Table 1.

Sequences of primers used for site-directed mutagenesis

OPG-Y49R5′-CCCTTGCCCTGACCACTACCGCACAGACAGCTGGCACACC-3′ Forward
5′-GGTGTGCCAGCTGTCTGTGCGGTAGTGGTCAGGGCAAGGG-3′ Reverse
OPG-N81A5′-GAGTGCAATCGCACCCACGCCCGCGTGTCG-3′ Forward
5′-GCATTCGCACACGCGGGCGTGGGTGCGA-3′ Reverse
OPG-F96A5′-GGGCGCTACCTTGAGATAGAGGCCTGCTTGAAACATAGGAGCTGC-3′ Forward
5′-GCAGCTCCTATGTTTCAAGCAGGCCTCTATCTCAAGGTAGCGCCC-3′ Reverse
OPG-F107A5′-GGAGCTGCCCTCCTGGAGCTGGAGTGGTGCAAGCTGG-3′ Forward
5′-CCAGCTTGCACCACTCCAGCTCCAGGAGGGCAGCTCC-3′ Reverse