Table 2.

Verapamil, cyclosporin A, and resveratrol enhanced ursolic acid-mediated cytotoxicity in Ca3/7 cells, indicated by a decrease in the IC50 for ursolic acid (average of 3–4 independent experiments, n = 3/exp)

CompoundDoseFold decrease of IC50P
+Veh1
+5 μmol/L1.22 ± 0.1<0.01
Verapamil+10 μmol/L1.22 ± 0.13<0.05
+25 μmol/L1.86 ± 0.32<0.05
+50 μmol/L1.85 ± 0.15<0.0001
+100 μmol/L2.85 ± 0.41<0.005
+Veh1
+5 μmol/L1.34 ± 0.06<0.005
Cyclosporin A+10 μmol/L1.53 ± 0.2<0.05
+25 μmol/L1.82 ± 0.27<0.05
+50 μmol/L1.96 ± 0.43<0.05
+100 μmol/L1.93 ± 0.39<0.05
+Veh1
+5 μmol/L0.91 ± 0.16
Resveratrol+10 μmol/L1.0 ± 0.29
+25 μmol/L1.35 ± 0.15<0.01
+50 μmol/L1.42 ± 0.17<0.005
+100 μmol/L2.31 ± 0.52<0.01

NOTE: The cytotoxicity of ursolic acid was enhanced by verapamil, cyclosporin A, and resveratrol in Ca3/7 cells.