Table 1.

miRNA expression and Ct values in untreated cells

1–5 μmol/L LPA induction (1 h)
Cell typemiR-30c-2* CtmiR-30c CtmiR-30c-2* CtmiR-30c Ct
OVCAR-328.39 ± 3.4222.16 ± 0.2120.2521.23
SKOV-327.40 ± 2.4719.78 ± 0.8024.4619.23
Hey-A8 MDR24.52 ± 0.14N/DN/DN/D
A54930.89 ± 0.2819.84 ± 0.90N/DN/D
IOSE27.63 ± 0.1017.38 ± 0.02N/DN/D

NOTE: For different cell types, the raw Ct values are presented as the average ± SEM and reflect 3 to 6 repeated experiments done using triplicates. For the 5 micromolar induction with LPA for 1 hour, this value reflects the result produced from the high-throughput microRNA screening experiment done in OVCAR-3 cells.

Abbreviation: N/D, no data.