Table 1.

Correlation between expression of RASSF1A and its promoter methylation status in uveal melanoma tumors

PatientsChromosome 3 statusDNA methylation RASSF1A promotor statusRelative RASSF1A mRNA expression
UMU/M
HealthyDisomy+1
1Disomy+0
2Disomy+0
3Disomy+0.4
4Disomy+0.3
5Disomy+0
6Disomy+0
7Monosomy+0
8Monosomy+0
9Monosomy+0
10Monosomy+0
11Monosomy+0
12Monosomy+0
13LOH−+0.2
14LOH−+0.9
15LOH−+0.6
16LOH−+0.3
17LOH + (D3S1478)+0.3
18LOH +(D3S1478)+0.1
19LOH + (D3S1478)+0
20LOH + (D3S1478)+0.6
21Microsatellite alterations+0

NOTE: Chromosome 3 status was determined by FISH in healthy tissue from human enucleated eyes and in tumors of patients 1 to 12. The microsatellite markers, D3S1478 and D3S1578, located on chromosome 3p21.3 and flanking the RASSF1A locus, were used to analyze the LOH on 9 uveal melanoma tumors (13–21). The methylation of RASSF1A promoter was analyzed by MSP on PCR products with specific primers from bisulfite-treated genomic DNA. The expression level of RASSF1A was analyzed by qPCR on total RNA available.

Abbreviations: LOH−, no deleted locus; LOH+, deleted 3p21.3 locus; M, Methylated; U, Unmethylated.