Table 1.

Parameters characterizing the movement of rhabdomyosarcoma RD, RH5, CW9019, RH28, and RH30 cells moving in control medium in the presence of SDF-1 (300 ng/mL) and in the presence of SDF-1 (300 ng/mL) with thrombin (5 U/mL)

Cell lineTLcTAScLTLcDAVcDCME
RD
    Control102.39 ± 16.570.43 ± 0.0618.10 ± 14.490.07 ± 0.060.19 ± 0.17
    SDF112.97 ± 21.16*0.47 ± 0.08*32.77 ± 24.47*0.13 ± 0.10*0.30 ± 0.2*
    SDF + thrombin106.25 ± 16.310.44 ± 0.0611.81 ± 7.760.04 ± 0.030.11 ± 0.08
RH5
    Control113.91 ± 33.100.47 ± 0.1315.33 ± 6.980.06 ± 0.030.15 ± 0.10
    SDF111.31 ± 19.120.46 ± 0.0718.14 ± 11.190.07 ± 0.040.16 ± 0.10
    SDF + thrombin127.33 ± 18.400.53 ± 0.076.67 ± 4.91*0.03 ± 0.02*0.05 ± 0.04*
RH28
    Control102.45 ± 26.710.42 ± 0.1112.95 ± 8.820.05 ± 0.030.14 ± 0.10
    SDF107.24 ± 19.520.44 ± 0.0818.45 ± 10.50*0.08 ± 0.04*0.17 ± 0.12
    SDF + thrombin120.31 ± 23.03*0.5 ± 0.09*16.26 ± 10.660.07 ± 0.040.15 ± 0.13
RH30
    Control108.81 ± 12.180.45 ± 0.0526.93 ± 17.620.11 ± 0.070.24 ± 0.16
    SDF149.00 ± 20.00*0.62 ± 0.08*38.13 ± 20.04*0.17 ± 0.08*0.31 ± 0.05
    SDF + thrombin122.99 ± 17.18*0.51 ± 0.07*45.98 ± 24.75*0.18 ± 0.10*0.37 ± 0.19*
CW9019
    Control122.21 ± 27.870.50 ± 0.1114.09 ± 9.650.06 ± 0.040.12 ± 0.10
    SDF105.96 ± 15.98*0.44 ± 0.06*21.05 ± 17.03*0.08 ± 0.07*0.21 ± 0.19*
    SDF+THROMBIN105.27 ± 20.24*0.44 ± 0.08*6.32 ± 6.21*0.02 ± 0.02*0.06 ± 0.07*

NOTE: Values are given as the mean ± SEM.

Abbreviations: TLcT, total length of cell trajectory (μm); AScL, average speed of cell locomotion (μm/min) is defined as the total length of the cell trajectory/time of recording (240 min); TLcD, total length of cell displacement (μm); aVcD, average velocity of cell displacement (μm/min) is defined as the length of the final cell displacement/time of recording; CME, coefficient of movement efficiency is the ratio of cell displacement to cell trajectory length. CME would equal 1 for a cell moving persistently along one straight line in one direction and 0 for a random movement.

  • *Statistically significant vs control.

  • Statistically significant vs SDF at P < 0.05 (Mann-Whitney test).