Table 2.

Q-PCR analysis of EpoR mRNA expression in a range of cancer cell lines, with conversion to copy number per 10 ng RNA

CellsCell typeCT valueEpoR copy number% compared with erythroid progenitors
Rama 37Rat mammary epithelial>35N/DN/D
Rama 37-50Rat mammary epithelial22.8 ± 0.148,595 ± 1,30328
Rama 37-28Rat mammary epithelial19.28 ± 0.43133,652 ± 59,987435
BFU-EHuman erythroid progenitors21.16 ± 0.3430,753 ± 8,408100
MDA-MB-435SHuman mammary epithelial25.03 ± 0.351,608 ± 4295
MDA-MB-231Human mammary epithelial25.39 ± 0.231,205 ± 2054
MCF7Human mammary epithelial25.68 ± 0.11951 ± 753
MCF10AHuman mammary epithelial27.17 ± 0.32310 ± 771
MCF10ATHuman mammary epithelial26.02 ± 0.30745 ± 1572
MCF10A-CA1aHuman mammary epithelial28.64 ± 0.59104 ± 460.3
C9Human cervical epithelial>35N/DN/D
H838Human non–small cell lung carcinoma26.30 ± 0.28603 ± 1342
H157Human non–small cell lung carcinoma25.31 ± 0.331294 ± 3094
H23Human adenocarcinoma25.82 ± 0.29870 ± 1803
UT7Human erythroleukemia (AML-M6)21.91 ± 0.8319,893 ± 10,64265
K562Human chronic myeloid leukemia25.51 ± 0.691,210 ± 5194

Abbreviation: N/D, nondetectable using 10 ng RNA template.