Table 1.

Cycle threshold (Ct) values from vascular cells used in this study

GeneHUVECHUAECMVEC
ATX32.9 ± 0.2435.3 ± 0.1435.9 ± 0.15
VEGF27.5 ± 0.1934.1 ± 0.1226.3 ± 0.43
VEGFR133.5 ± 0.2328.2 ± 0.1923.5 ± 0.40
VEGFR230.1 ± 0.2128.1 ± 0.0621.8 ± 0.50
S1P119.6 ± 0.0820.8 ± 0.4122.2 ± 0.26
S1P225.0 ± 0.1027.1 ± 0.1521.7 ± 0.13
S1P336.8 ± 0.5233.3 ± 0.6534.1 ± 0.41
LPA125.3 ± 0.01>38>38
LPA225.3 ± 0.11>38>38
LPA331.3 ± 0.24>38>38
HPRT121.0 ± 0.0622.1 ± 0.1120.7 ± 0.05

NOTE: Average Ct ± SD; Ct > 38 is beyond the instrument range. HUVEC, HUAEC, and MVEC were cultured under standard conditions for 20 h, and total RNA was assayed for ATX, VEGF, VEGFR1, VEGFR2, S1P1, S1P2, S1P3, LPA1, LPA2, and LPA3 expression using qRT-PCR. HPRT1 served as an internal control in qRT-PCR calculations.