Table 1.

Nuclear localization of HPSE in BMBL is associated with HER2 overexpression

HER2 classificationHPSE nucleolar staining*Cells with HPSE/Topo I overlay
Overexpressed9/11 (81.8%)72%
Negative3/17 (17.6%)5%

NOTE: Human breast cancer specimens were analyzed as described in Materials and Methods.

  • *Nucleolar staining was determined by fibrillarin staining. Samples percentage represents HPSE nucleolar staining/total number of samples assayed. Nucleolar staining was considered positive only when HPSE/Fibrillarin staining colocalized in >60% of the total cells. Eight to 10 fields were counted for each experiment and were averaged. Overlay of HPSE/Fibrillarin percentage was determined by comparison with Fibrillarin staining only.

  • Eight to 10 fields were counted for each slide and averaged. Overlay of HPSE/Topo I percentage was determined by comparison with Topo I staining only.