Table 1.

qRT-PCR Oligonucleotide Primers Specific for the Eight Conserved Regions in ERBB2 Gene and an Irrelevant Genomic Control Region Analyzed by Chromatin Immunoprecipitation

ERBB2 geneOrientationSequencePCR Product Size (bp)
CR1Forward5′-ATCCTCTCCCTGCTCACCTC-3′164
Reverse5′-GGCTACTTCTTACTCATTCCAACC-3′
CR2Forward5′-TGGTGGAAGTGGGAGTAGAGA-3′223
Reverse5′-TGAAGCCAAATACAAGTTAGGAAG-3′
CR3Forward5′-GTTCCTCGTCTCCTCTTCCTTG-3′324
Reverse5′-TAGCTTTGCTTTGCCACCTG-3′
CR4Forward5′-CTGCCCCTTTGCTGTCCT-3′167
Reverse5′-AGGCTTGAGGTGTCCCTTTG-3′
CR5Forward5′-ATCTCAAGGCTCAAGGTTCCTC-3′323
Reverse5′-TCCAGGAGTCACTGGTTCATC-3′
CR6Forward5′-AGCACATGGAAGCAAGTTTAG-3′163
Reverse5′-CCCAGCCAAGAATGCAG-3′
CR7Forward5′-TCCTTTCGATGTGACTGTCTCC-3′339
Reverse5′-CTAAATGCAGAGGCTGGTGACT-3′
CR8Forward5′-GCTTAGGGACTGTGCTCTGTG-3′220
Reverse5′-TGGACAGATGGGTCAGGATAC-3′
ControlForward5′-ATGGTTGCCACTGGGGATCT-3′174
Reverse5′-TGCCAAAGCCTAGGGGAAGA-3′