Table 2.

Sequences and Amplicon Sizes of the Primers

Primer nameSequence
HPRT amplicon size (94 bp)
    HPRT forward5′-AGGAAAGCAAAGTCTGCATTGTT-3′
    HPRT reverse5′-GGCTTTGTATTTTGCTTTTCCA-3′
GAPDH amplicon size (87 bp)
    GAPDH forward5′-CCACATCGCTCAGACACCAT-3′
    GAPDH reverse5′-AAAAGCAGCCCTGGTGACC-3′
IL-8 amplicon size (151 bp)
    IL-8 forward5′-GCCTTCCTGATTTCTGCAGC-3′
    IL-8 reverse5′-CGCAGTGTGGTCCACTCTCA-3′
IL-6 amplicon size (104 bp)
    IL-6 forward5′-AGCCAGAGCTGTGCAGATGAG-3′
    IL-6 reverse5′-TGGCATTTGTGGTTGGGTC-3′

NOTE: Selecting suitable intron-spanning primers, cDNA amplicon was much shorter than genomic DNA amplicon. Excluding falsification by amplification of possible pseudogene sequences, both primer sets were shown to detect no genomic DNA with cDNA sequence. The primer sets were selected to work under identical PCR cycling conditions, so that simultaneous amplification of HPRT, GAPDH, IL-6, and IL-8 was obtained in the same run.