Table 3.

Cloning and Sequencing Primers Used in This Study

Exons TargetedPrimerSequence (5′→3′)Nucleotides Targeted
Primers for cloning exons 8-13 and 17-19
    8-111159SGGGAACGAAGGAGGCACTTACACA1159-1182
1843ASCATCCGACTTAATCAGGCCATA1821-1843
    11-131651SATGTATGAAGTACAGTGGAAGG1651-1672
2131ASAAGTGCCACTTCTCCGTGATC2083-2103
    17-192321SCTAGAGGACTTGCTGAGCTT2293-2312
2721ASATCCCAGCACGTCTTCATGA2702-2721
Primers for sequencing exons 8-13 and 17-19
    8-91159SGGGAACGAAGGAGGCACTTACACA1159-1182
1494ASGTTGTAAGCCCTACACTCGAC1471-1494
    9-111336SGTCCAGGAGCTGAGCAGAG1137-1159
1843ASCATCCGACTTAATCAGGCCATA1821-1843
    11-131651SATGTATGAAGTACAGTGGAAGG1651-1672
2131ASAAGTGCCACTTCTCCGTGATC2083-2103
    17-192321SCTAGAGGACTTGCTGAGCTT2293-2312
2721ASATCCCAGCACGTCTTCATGA2702-2721
Primers for full-length cloning using the Gateway system
    1-21GWATG senseggggacaagtttgtacaaaaaagcAGGCTATCGCAGCCACCGCGATGAG−17 to +5
GWRggggaccactttgtacaagaaagctgggtGATCGCTCTTGTTGGGGAGAC2948-2968
  • NOTE: Nucleotides targeting the canine KIT sequence (Genbank accession number NM001003181) are shown in uppercase letters. Nucleotides added to the PCR primers for cloning using the Gateway system are shown in lowercase letters. Underlined letters indicate the predicted start (ATG) codon.