NF-κB–Deficient Primary MEFs Undergo Normal Cell Cycle Arrest in Response to Doxorubicin Treatment
BrdUrd | wt | c-rel−/− | rela−/− | nfkb1−/−rela−/− | c-rel−/−rela−/− | p53−/− |
---|---|---|---|---|---|---|
Medium | 17.8 ± 4 | 13.9 ± 0.4 | 14.1 ± 0.9 | 9.2 ± 0.5 | 14.7 ± 0.8 | 35.5 ± 1.5 |
200 ng/mL doxorubicin | 0 | 0.1 ± 0 | 0 | 0.2 ± 0.2 | 0 | 11.8 ± 1.1 |
NOTE: wt, NF-κB–deficient, and p53-deficient MEFs were treated with 200 ng/mL doxorubicin for ∼20 h. Cells were then pulsed with BrdUrd for 5 h, fixed, incubated with FITC-conjugated anti-BrdUrd antibody, and analyzed by fluorescence-activated cell sorting. The percent of BrdUrd-positive MEFs represents the percentage of cells in the S phase of the cell cycle. The values represent the mean ± SD of duplicates from two independent experiments.