Table 2.

NF-κB–Deficient Primary MEFs Undergo Normal Cell Cycle Arrest in Response to Doxorubicin Treatment

BrdUrdwtc-rel−/−rela−/−nfkb1−/−rela−/−c-rel−/−rela−/−p53−/−
Medium17.8 ± 413.9 ± 0.414.1 ± 0.99.2 ± 0.514.7 ± 0.835.5 ± 1.5
200 ng/mL doxorubicin00.1 ± 000.2 ± 0.2011.8 ± 1.1
  • NOTE: wt, NF-κB–deficient, and p53-deficient MEFs were treated with 200 ng/mL doxorubicin for ∼20 h. Cells were then pulsed with BrdUrd for 5 h, fixed, incubated with FITC-conjugated anti-BrdUrd antibody, and analyzed by fluorescence-activated cell sorting. The percent of BrdUrd-positive MEFs represents the percentage of cells in the S phase of the cell cycle. The values represent the mean ± SD of duplicates from two independent experiments.