Table 2.

Primers Used in the Construction of hPygo2 Promoter Constructs

ConstructForward PrimerReverse Primer
pGL3(−1494)/(as-1494)5′-CGGTTTGGGTTGGGATAACAGAGG5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−1331)5′-GTATATACATTTCATAAGGCCTGCC5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−1143)5′-GCATTGAGACTATAAAGCAGTAGAC5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−829)5′-GGTCTCTTACCGCGAGACTGAG5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−533)5′-CTTCTGGGAAGCCTATTGTGTACGCC5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−164)5′-CCTTTCCACTCGCGTGGTGCCGTG5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−108)5′-CAGGCGTAGCGTCTCGTCCGGTC5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−48)5′-CGCGACGAGTTCCGGTTCCG5′-CCGAGCTGCAGCAACCACAAAGTG
pGL3(−48) Ets A5′-GATCTCGCGACGAGTTCAGGTTCCGGTTGCTGCC5′-GGCAGCAACCGGAACCTGAACTCGTCGCGAGATC
pGL3(−48) Ets B5′-GCGACGGAGTTCCGGTTCAGGTTGCTGCCGCCG5′-CGGCGGCAGCAACCTGAACCGGAACTCGTCGC
pGL3(−48) Ets A+B5′-GATCTCGCGACGAGTTCAGGTTCAGGTTGCTGCCGCCG5′-CGGCGGCAGCAACCTGAACCTGAACTCGTCGCGAGATC