Table 1.

Top Priority Genes for Methylation in Primary ESCC by CVV Score

AccessionNameN1 + N2C1C2C3CVVMethylation*Derived from
1X76220MALPDDD226.8M1st
2U51712HOP/OB1/NECC1PDDD158.2M2nd
3ALO38340Crystallin αBPDNCI130.3M1st
4J0285420-kDa myosin light chain (MLC-2)PDDD100.6U1st
5L76465PGDHPDDD68.9U2nd
6AB014533KIAA0633 (COBL)PDDD45.9M1st
7U10550GemPDDD38.2U1st
8AB011143KIAA0571 (GRB2-associated binding protein 2)PDDD36.9U2nd
9J02611Apolipoprotein DPDDD37.1(M)1st
10D83402Prostacyclin synthasePDDD35.62nd
11AF032108Integrin α7PDDD34.4M1st
12AFO39397Crytallin-μPDNCD26.1U2nd
13M55153Transglutaminase-2PDDD25.7(M)2nd
14U22398Kip2/p57PDNCNC22.6M1st
15X71345Trypsinogen IVbPDDD19.2(M)2nd
16U68385MEIS3PINCD18.21st
17U35139NecdinPDDD17.51st
18AL035494FLJ10097 homology with p75NTR-associated cell death executorPDDD16.3M2nd
19M96740NSCL-2PDINC15.51st
20AJ012737Filam inPDDD14.82nd
21J00073Actin, α, cardiac muscle; actinPDNCD13.32nd
22J03910Metallothionein 1GPDDD12.3(M)1st
23NM_002026FibronectinPDNCNC10.62nd
24L08044Intestinal trefoilfactorPDDD9.7M1st
25D50918KIAA0128 (septin 6)PNCDD9.62nd
26AF020202Munc-13PDDD9.0M1st
27AL049949DKFZp564 (FLJ90798)PDDD8.7M1st
28U29091Selenium binding proteinPDNCD8.4(M)2nd
29AB018264KIAA0711 (TSPY-like 4)PDDD8.2(M)1st
30U85707MEIS1PDNCD7.71st
31X56667CalretininPDID6.42nd
32J05582Mucin-1PDDD6.32nd
33X51405Carboxypeptide EPDDD5.72nd
34M84526AdipsinPDDD5.2(M)1st
  • Abbreviations: N, normal tissue; C, cancer tissue; P, present in expression; D, decrease; I, increase; NC, no change.

  • * Methylation in ESCC cell lines was evaluated by direct sequencing. M, methylation; U, unmethylation; —, not assessed in this study; (), was examined in previous studies (5, 8).