Table 3.

SDF-1 Prevents TNFα–Mediated Apoptosis

TNFα-treated cellsTNFα-treated cells incubated with SDF-1
Annexin V–negative cells (flow cytometry), %93.1 ± 698.9 ± 3*
Annexin V–positive cells (flow cytometry), %6.8 ± 0.51.1 ± 0.3*
  • NOTE: Cells were serum deprived for 24 h, incubated for 48 h in medium supplemented with TNFα (50 nmol/L), and coincubated or not with SDF-1α (125 nmol/L) for 48 h. Cell staining with Annexin V was analyzed by flow cytometry. Data are mean ± SE of three independent experiments.

  • * P < 0.05, versus SDF-1α–untreated cells.