Table 2.

SDF-1 Prevents Huh7 Cell Spontaneous DNA Degradation and Stimulates the Transition of Cells Already Engaged in G1 to S + G2-M

SDF-1 treatment% Cells in cell cycle phases (mean ± SD)
Sub-G1G0-G1S + G2-M
51.5 ± 9.428.7 ± 8.419.8 ± 4.8
+25.0 ± 6.8*39.4 ± 7.2*35.6 ± 6.9*
  • NOTE: Cells were serum deprived for 24 h and incubated for 48 h in medium supplemented or not (control) with SDF-1α (125 nmol/L). Cells were pretreated with RNase, stained with propidium iodide, and analyzed by flow cytometry. The cell histogram FL-2 was divided into three regions according to the cell cycle phases, sub-G1, G0-G1, and S + G2-M. Data are mean ± SE of three independent experiments.

  • * P < 0.05, versus untreated cells.