Table 1.

SDF-1 Triggers Quiescent Huh7 Cells from G0 into Cycle

Untreated cellsSDF-1 stimulated cells
Proliferative labeling index: Ki67/DAPI positive cells (immunocytochemistry), %75 ± 495 ± 1*
Ki67 fluorescence intensity (flow cytometry)7 ± 0.521 ± 1.2*
  • NOTE: Expression of Ki67 proliferation–associated nuclear antigen was determined by immunocytochemistry and flow cytometry, as indicated. The proliferative labeling index was as follows: (number of cells with moderate to strong nuclear reactivity for anti-Ki67 mAb / the number of 4′,6-diamidino-2-phenylindole (DAPI)–positive cells) × 100. The Ki67 fluorescence intensity was the difference between the mean fluorescence intensity of the cells labeled with anti-Ki67 mAb and that of the cells labeled with the isotype IgG1. Data are mean ± SE of at least three independent experiments.

  • * P < 0.05, versus untreated cells.