Table 1.

Cell Populations in Cultures of Normal Fibroblasts and Melanomas under Control and MDS Conditions as a Function of Time

Cell lineDays under MDS
12345610
CCD-187sk103*1059492939591
E.DERM99949294919095
M59195873223212118
M25591822211997
M1088541199111111
M10391451512889
M105102918483474313
WM3599937545312811
A37511192662823198
MEWO93782813121311
  • NOTE: Cells were plated in 27 μmol/L methionine-efficient medium in 10% dialyzed serum. Cell numbers were monitored daily, and when cultures reached confluence, methionine was replaced with 27 μmol/L homocysteine thiolactone. Medium was replaced every 36 hours. Controls were supplied with methionine and 10% dialyzed serum every 36 hours throughout the experiment. Cells were counted at five different fields every 24 hours and the median number was expressed as percent of cells in control cultures. Time period of massive apoptosis is shown in bold for each culture.

  • * Median of 15 determinations (3 plates, 5 fields each) showing change in cell numbers as percent of control (methionine-efficient medium).