Table 3.

Immunofluorescence Flow Cytometry Determination of Cell Surface Expression of TACSTD2 and ERBB3

Cell lineIC50 (μmol/L)TACSTD2
ERBB3
% PositiveMedian fluorescence intensityArray% PositiveMedian fluorescence intensityArray
Training set
    H3580.1896218,895996.4554
    H322C0.25943410,421965.2685
    Calu-30.399357,141864.41,444
    H16480.38982514,348691.8561
    H1254.8100674947.59
    H17038.0004006
    A5499.6004423.951
    H15712.8004005
    H46012.9004005
    H52013.6004005
Test set
    HCC780.41002919,788999.54,027
    HCC1931.51005913,425984.9200
    HCC951.9833.94,461742.21,060
    HCC447.9893.5316009
    H20098.9992911,9808661,246
    HCC159.4271.5940791.7141
    H129914.7004361.99
EGFR mutant
    HCC8270.00594243,769652.7384
    H32550.0151002811,334906.41,931
    HCC40060.0294207,647851.8538
    H22790.0300703005
    H16501.0952313,196843.698
    H8203.099307,338953.9504
    H19758.046382,329393.8197