Table 1.

Analysis of Oxytocin-Induced Migration of HMEC and B-TEC Migration Assay Was Done in the Boyden Chamber by Adding Oxytocin or VEGF in the Lower Compartments of the Chamber to Establish Positive or Absent Gradient across the Filter Barrier

Lower chamberHMEC no. migrated cellsP vs vehicleB-TEC no. migrated cellsP vs vehicle
Vehicle34 ± 232 ± 2
VEGF121 (20 ng/mL)604 ± 32<0.001748 ± 49<0.001
Anti-VEGF antibody (50 ng/mL)25 ± 724 ± 5
VEGF121 (20 ng/mL) + anti-VEGF antibody (50 ng/mL)80 ± 1286 ± 17
Oxytocin (10 nmol/L)524 ± 29<0.001816 ± 30<0.001
Oxytocin (1 nmol/L)276 ± 15<0.005543 ± 20<0.001
Oxytocin antagonist (10 nmol/L; upper chamber) + oxytocin (10 nmol/L)40 ± 252 ± 7
  • NOTE: Cells that migrated after 12 hours of incubation to the lower surface of the filter were counted. Preincubation with a selective oxytocin antagonist or anti-VEGF antibody was done in additional experiments to further test the specificity of the used stimuli. Numbers are mean ± SD of one representative experiment done in triplicate and counted by two independent investigator.