Table 2.

Effect of the Antisense Oligonucleotide to VKOR on VKOR Activity and Expression

VKORRelative Enzyme ActivityRelative Amount of mRNA
Control1001.0
Antisense (20.0 μmol/L)460.5
Sense (20.0 μmol/L)900.9
Mutant antisense (20.0 μmol/L)880.9
  • NOTE: HUVECs were treated with the antisense oligonucleotide to VKOR (20.0μmol/L) for 1 day. VKOR activity was measured as the ratio of the percentage of substrate VKOR converted into quinine. The mRNA of VKOR was analyzed by real-time PCR, with β-actin as a control. All tests were done in duplicate.