Table 2.

Genes Whose Deletion Cause Sensitivity to UPR-Inducing Agents

Gene NameORFProtein FunctionSensitivity
TunicamycinDTTβ-Mercaptoethanol
SLT2 MAPK pathway
    SLT2YHR030CCell wall organization and biogenesis, response to stress and nitrogen starvationSSSSSSSSS
    BCK1YJL095WCell wall organization and biogenesis, response to stress and nitrogen starvationSSSSSSSSS
    RLM1YPL089CTranscription factor for cell wall organization and biogenesisSSSWT
    HCS77YOR008CPlasma membrane protein for maintenance of cell wall integritySSSSWT
    MID2YLR332WPlasma membrane protein for maintenance of cell wall integritySSSS
    MKK2YPL140CMAPK kinaseSWTS
    MKK1YOR231WMAPK kinaseSWTS
Osmosensing MAPK pathway
    SSK22YCR073CMAPK kinaseWTSSS
    SSK1YLR006COsmosensory signaling pathway via two-component systemSSSS
    PBS2YJL128CProtein threonine/tyrosine kinaseSSS
    HOG1YLR113WMAPK of the high-osmolarity signal transduction pathwaySSSS
    SSK2YNR031CMAPK kinase kinaseSWTS
Calcium channel
    PMR1YGL167CCalcium-transporting ATPase, ER-to-Golgi transportSSSSSS
    SPF1YEL031WCalcium-transporting ATPaseSSSSWT
    CNB1YKL190WCalcineurin regulatory (B) subunitSSSSSS
UPR
    IRE1YHR079CResponse to unfolded proteinSSSSSSSSS
    HAC1YFL031WTranscription factor that activates UPRSSSSSSSSS
Protein glycosylation
    RHK1YBL082Cα-1,3-Mannosyltransferase activityWTSS
    ALG9YNL219CProtein amino acid glycosylationSSSS
    KTR7YIL085CGlycoprotein biosynthesisSSSS
    OST3YOR085WN-linked glycosylation protein amino acid glycosylationWTSSS
    ALG8YOR067CGlucosyltransferase of the ERWTSSS
Transcription
    ISW2YOR304WTranscriptional repressorWTSS
    RIM101YHL027WSpecific transcriptional repressorSSS
    MSN4YKL062WTranscription factor that response to stressSWTWT
    PHO80YOL001WCyclin-dependent protein kinaseSSWTWT
    DAL81YIR023WSpecific RNA polymerase II transcription factorSWTSS
    PIP2YOR363CDNA binding, transcriptional activator activityWTSS
    ARD1YHR013CRegulation of transcription, mating-type specificWTSSS
Transport
    FPS1YLL043WGlycerol transporterSSSS
    DID2YKR035W-ANonselective vesicle transport, protein-vacuolar targetingSSSSS
    SEC28YIL076WVesicle-mediated transport, retrograde (Golgi-to-ER) transportWTSWT
    SLS1YOL031CER protein of unknown functionSSWT
    TAT2YOL020WAromatic amino acid transporter activityWTSSS
    ERV25YML012WER-to-Golgi transportSWTS
    EDE1YBL047CPolar budding, endocytosis, protein transportWTWTS
    UGO1YDR470cMitochondrial outer membrane; transporterWTWTSS
    VPS30YPL120WVesicle-mediated transport, protein-Golgi targetingSWTSS
Ungrouped or unknown genes
    RGA1YOR127WCytokinesis, regulation of cell shapeWTWTSS
    RMD1YDL001WProtein possibly involved in meiotic nuclear divisionWTSSSS
    RGD1YBR260CRho GTPase activator, establishment and/or maintenance of cell polaritySSSWT
    SPC2YML055WComponent of the signal peptidase complexSSSWT
    SWE1YJL187CProtein tyrosine kinaseSSSS
    FYV8YGR196CProtein of unknown functionSSSSS
    SEL1YML013WProtein involved in secretionSSWTS
    YKR035CYKR035CProtein of unknown functionSSSSSS
    YMR007WYMR007WGlucosyltransferase of the ERSSSWT
    YMR295CYMR295CProtein of unknown functionSWTWT
    YMR294W-AYMR294W-AProtein of unknown functionSWTWT
    VAM10YOR068CProtein of unknown functionWTSS
    YOR093CYOR093CProtein of unknown functionSRS
    SRC1YML034WProtein of unknown functionSWTWT
    SPS4YOR313CProtein of unknown functionSWTWT
    YBL083CYBL083CProtein of unknown functionSSS
  • NOTE: Individual sensitivities of deletion mutants to ER stress organized by functional categories. Each mutant is ranked by relative sensitivity to UPR-inducing agents. For example, IRE1 is classified as “SSS” where “S” indicates impaired colony-forming ability that corresponds to one dilution (∼10-fold), “SS” corresponds to two dilutions and “SSS” to three or more dilutions. “R” refers to a resistant phenotype and “WT” indicates the same sensitivity as the wild-type. All references to the primary data and the known function of the proteins are given in the proteome database (https://www.incyte.com/proteome/YPD).