TABLE 1.

Sodium Butyrate and H-7 Show Antagonistic Effects on mRNA Levels of TJ Proteins in Rat-1 Fibroblasts

ΔCt
Fold Change
Control+ Sodium Butyrate+ H-7+ Sodium Butyrate + H-7Sodium Butyrate versus ControlSodium Butyrate + H-7 versus Sodium ButyrateH-7 versus Control
Cingulin15.9 ± 0.114.4 ± 0.117.9 ± 0.317.1 ± 0.3↑ 2.8↓ 6.5↓ 4.0
ZO-113.3 ± 0.212.0 ± 0.113.8 ± 0.215.8 ± 0.2↑ 2.5↓ 13.9↓ 1.4
ZO-215.2 ± 0.114.3 ± 0.221.4 ± 0.219.2 ± 0.2↑ 1.9↓ 30.1↓ 73.5
  • NOTE: ΔCt values represent the difference between the Ct value of the indicated gene and the Ct value of the 18S RNA gene used as an internal control. Relative increases/decreases (fold change) in mRNA levels are indicated by arrows and were calculated according to the formula 2−(ΔΔCt). Note that sodium butyrate up-regulates mRNA levels, whereas H-7 down-regulates mRNA levels and reverses the effect of sodium butyrate.