Table 1.

Effect of Antisense CK2α Oligodeoxynucleotide on Cell Viability of Cancer and Noncancer Cells in Culture

Antisense CK2α OligodeoxynucleotideTime (h)% Cell Viability ± SE*
ALVA-41PC3-LN4BPH-1PrECNormal Human Dermal FibroblastsNormal Human Epidermal Keratinocytes
2 μg/mL Nonsense24100 ± 8100 ± 16100 ± 3100 ± 10100 ± 8100 ± 14
2 μg/mL Antisense2474 ± 1080 ± 1296 ± 5100 ± 12100 ± 12100 ± 7
5 μg/mL Nonsense24100 ± 16100 ± 14100 ± 11100 ± 8100 ± 9100 ± 12
5 μg/mL Antisense2459 ± 1469 ± 1194 ± 8100 ± 1498 ± 11100 ± 6
2 μg/mL Nonsense48100 ± 14100 ± 18100 ± 10100 ± 16100 ± 9100 ± 17
2 μg/mL Antisense4868 ± 670 ± 1694 ± 9100 ± 990 ± 9100 ± 12
5 μg/mL Nonsense48100 ± 5100 ± 16100 ± 6100 ± 17100 ± 11100 ± 15
5 μg/mL Antisense4842 ± 464 ± 1183 ± 2100 ± 14.5pt>90 ± 5100 ± 16
  • * Cells were plated in 96-well plates and transfected with the antisense CK2α oligodeoxynucleotide or the nonsense oligodeoxynucleotide control at the concentrations indicated and for the times shown. Cell viability was determined using WST-1 reagent as described in Materials and Methods. Data are expressed as percentage ± SE cell viability and are based on three experiments.