Table 5.

Overexpression of a Dominant Negative p110α (Δp110α) Restores Sensitivity of HL-60 AR Cells to Drugs and ATRA

TreatmentMTa7E7M9B
None (control)1.7 ± 1.35.2 ± 2.66.1 ± 2.25.0 ± 2.3
Camptothecin (0.1 μg/ml)3.5 ± 2.155.0 ± 6.364.2 ± 6.860.1 ± 6.4
Etoposide (5 μg/ml)3.8 ± 1.939.5 ± 4.640.9 ± 4.543.7 ± 5.7
Doxorubicin (100 μg/ml)5.1 ± 2.825.0 ± 2.928.7 ± 2.826.4 ± 3.2
Cytarabine (40 μg/ml)5.5 ± 2.536.3 ± 3.841.4 ± 4.642.3 ± 5.0
Mitoxantrone (40 μg/ml)5.6 ± 2.826.6 ± 3.025.6 ± 3.927.4 ± 3.2
Vincristine (4 μg/ml)5.9 ± 2.735.1 ± 3.234.5 ± 4.031.3 ± 3.6
ATRA8.1 ± 5.276.3 ± 8.170.6 ± 8.477.7 ± 7.9
  • Note: The results show the percentage of apoptotic (in the case of drugs) or differentiated (in the case of ATRA) cells. Cells were exposed to the drugs for 24 h or to ATRA for 4 days. Data are the means ± SD from three separate experiments.

  • a MT, mock-transfected clone.