Table 2.

Affectors That Interact With MDM2

ProteinsConsequence of Interactionp53 DependencyPhysiological Interaction MeasuredReferences
p53MDM2 ubiquitinates p53 and induces degradation.Yes(3, 4, 87, 88)
p73MDM2 inhibits p73 activity without degradation.NoNo(89–92)
P63MDM2 stabilizes p63 and increases the transcriptional activity.NoNo(93)
RBMDM2 inhibits the ability of RB to inhibit E2F1 function.NoYes(69, 70)
MTBPMdm2 cancels MTBP-induced G1 arrest p53 independently.NoNo(94)
PMLMDM2 promotes PML nuclear exclusion and inhibits PML-dependent transactivation.NoNo(95)
E2F/DP1Mdm2 stimulates E2F/DP1 transcriptional activity and DNA synthesis, while Mdm2 can antagonize the apoptotic property of E2F.NoYes (DP1)(73, 74)
Sp1Mdm2 inhibits Sp1 DNA binding but RB restores it.NoYes(71, 72)
NumbMdm2 alters subcellular localization of Numb and accelerates the degradation.N.E.No(79, 80)
ARAkt/MDM2 targets AR ubiquitination and degradation.N.E.Yes(78)
P300/CBPMdm2 blocks the interaction of p53 and the co-activator p300/CBP.NoYes(75–77)
PCAFMDM2 inhibits PCAF-mediated p53 acetylation and activation.N.E.No(85)
HDACMDM2-HDAC1-mediated deacetylation of p53 is required for its degradation.NoYes(86)
L5/RNAMDM2 may have functions in ribosomal biogenesis or translational regulation.Complex with p53Yes(81–83)
DNA pol. εMDM2 stimulates the activity of DNA polymerase ε.NoNo(99, 100)
TAFII250MDM2 activates cyclin A promoter through complex with TAFII250.N.E.No(97, 98)
TBP/TFIIE small subunitThe interaction may inhibit basal transcriptional machinery.N.E.No(96)