Table 3.

Cell Cycle Analysis of Cells Overexpressing MnSOD Treated with a p38MAPK Inhibitor

Note: Cells were cultured for 48 h without FCS and incubated in FCS-containing medium for 1 h with either 10 μm of a p38MAPK inhibitor (SB, SB203580) or 25 mm of a ROS scavenger (NAC). Then, cells were irradiated with 5 Gy of X-ray (IR). Twenty-four hours later, cells were collected and fixed in 70% ethanol and treated with RNase A, and then stained with propidium iodide. DNA contents of the cells were analyzed by FACScaliber using Cell Quest and Modifit programs.

Distribution of Cells in Each Stage (%)
StageUntreated
IRSBSB + IRNACNAC + IR
0 h24 h
ControlG167.664.733.867.037.768.435.9
S16.212.44.112.74.911.55.2
(G1-S ratio4.25.28.25.37.75.96.9)
G2-M16.222.962.120.357.420.158.9
MnSODG170.369.961.671.538.969.539.3
S14.98.34.96.84.07.14.9
(G1-S ratio4.78.412.610.59.79.88.0)
G2-M14.821.833.521.757.123.455.8