Table 1.

List of primers used for the RT-PCR detection of MMPs and TIMPs

Primer namePrimer sequenceSize (bp)Annealing temp. Ta (°C)
F-MMP15′-TGGGAGCAAACACATCTGAC-3′56064
R-MMP15′-ATCACTTCTCCCCGAATCGT-3′
F-MMP25′-GCAGATGCCTGGAATGCCAT-3′50459
R-MMP25′-AGGGTTCTGTGAGCCACAGA-3′
F-MMP35′-GCAGTTTGCTCAGCCTATCC-3′21462
R-MMP35′-GAGTGTCGGAGTCCAGCTTC-3′
F-MMP85′-TCTGCAAGGTTATCCCAAGG-3′25158
R-MMP85′-TTGGTCCACTGAAGACATGG-3′
F-MMP95′-GCTATGGTTACACTCGGGG-3′53064
R-MMP95′-GCCATCTGCGTTTCCAAACC-3′
F-MMP105′-CAGAAGTTCCTTGGGTTGGA-3′59862
R-MMP105′-GGGGAGGTCCGTAGAGAGAC-3′
F-MMP135′-AGGAGATGCCCATTTTGATG-3′40558
R-MMP135′-GGAAGTTCTGGCCAAAATGA-3′
F-MT1-MMP5′-ACATTGGAGGAGACACCCAC-3′50462
R-MT1-MMP5′-GGCAGTGTTGATGGACGC-3′
F-TIMP15′-ATTCCGACCTCGTCATCAG-3′40456
R-TIMP15′-CGTCCACAAGCAATGAGTG-3′
F-TIMP25′-GACGTTGGAGGAAAGAAGGA-3′37159
R-TIMP25′-CGTTGGAGGCCTGCTTATGG-3′
F-TIMP35′-GGGTGTGTGGCATTGATGAT-3′42864
R-TIMP35′-ATGTACCGAGGCTTCACCAA-3′
F-TIMP45′-CCACTCGGCACTTGTGATTCG-3′48962
R-TIMP45′-CAGGTGCCGTCAACATGCTTC-3′
F-18S5′-GTTGTTGGAGCGATTTGTCTGG-3′34461
R-18S5′-AGGGCAGGGACTTAATCAACGC-3′

Abbreviations: F, forward primer; R, reverse primer; bp, base pairs.