PT  - JOURNAL ARTICLE
AU  - Larsen, Alice Bjerregaard
AU  - Pedersen, Mikkel Wandahl
AU  - Stockhausen, Marie-Thérése
AU  - Grandal, Michael Vibo
AU  - Deurs, Bo van
AU  - Poulsen, Hans Skovgaard
TI  - Activation of the EGFR Gene Target EphA2 Inhibits Epidermal Growth Factor–Induced Cancer Cell Motility
AID  - 10.1158/1541-7786.MCR-06-0321
DP  - 2007 Mar 01
TA  - Molecular Cancer Research
PG  - 283--293
VI  - 5
IP  - 3
4099  - http://mcr.aacrjournals.org/content/5/3/283.short
4100  - http://mcr.aacrjournals.org/content/5/3/283.full
SO  - Mol Cancer Res2007 Mar 01; 5
AB  - EphA2 overexpression has been reported in many cancers and is believed to play an important role in tumor metastasis and angiogenesis. We show that the activated epidermal growth factor receptor (EGFR) and the cancer-specific constitutively active EGFR type III deletion mutant (EGFRvIII) induce the expression of EphA2 in mammalian cell lines, including the human cancer cell lines A431 and HN5. The regulation is partially dependent on downstream activation of mitogen-activated protein kinase/extracellular signal–regulated kinase kinase and is a direct effect on the EphA2 promoter. Furthermore, EGFR and EphA2 both localize to the plasma membrane and EphA2 coimmunoprecipitates with activated EGFR and EGFRvIII. Ligand activation of EphA2 and EphA2 knockdown by small interfering RNA inhibit EGF-induced cell motility of EGFR-overexpressing human cancer cells, indicating a functional role of EphA2 in EGFR-expressing cancer cells. (Mol Cancer Res 2007;5(3):283–93)