Table 1.

Caspase-3 and Caspase-7 Activity of MTag Cells Treated with Celecoxib

TreatmentCaspase-3/7 Activity (Fluorescence Emission)
Vehicle1 × 106 ± 2.0 × 105
Celecoxib (20 μmol/L)2 × 106 ± 1.6 × 105
Celecoxib (40 μmol/L)4 × 106 ± 2.2 × 105*
Celecoxib (60 μmol/L)5 × 106 ± 2.0 × 105*
  • NOTE: Spectrofluorometric analysis of lysates prepared from 48-hour vehicle-treated and celecoxib (20, 40, and 60 μmol/L)–treated MTag cells. Activity of caspase-3 and caspase-7 was monitored by enzymatic cleavage using a fluorescence microplate reader with excitation at 485 ± 10 nm and emission detection at 530 ± 12.5 nm. Mean ± SD of three experiments.

  • * P < 0.01, significant difference between vehicle control and celecoxib treatment.