To visualize transcription from the extrachromosomal double minutes in live cells, human COLO 320DM cells were transfected with a mixture of two plasmid. These two sequences, which appear as red and green in this image among the mitotic chromosomes (blue), were co-amplified at the double minutes, because one of the plasmid had IR/MAR sequence. In live cells, the plasmid sequences were visualized by specific binding to the cyan-fluorescence protein, while RNA transcribed from the plasmid was visualized by specific binding to the yellow-fluorescence protein. For details, see the article by Utani and colleagues on p. 695.

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