ABI-1 down-regulated MDA-MB-231 (not shown) and control cells (shown) were plated on a fibronectin pre-coated glass coverslip in media free of FCS for 18 hours. Cells were fixed with 3.7% formaldehyde/PBS solution and permeabilized with 0.1% Triton X-100 in PBS for 5 min. Next, cells were stained for F-actin with Rhodamine-coupled phalloidin for 30 min and mounted to standard slides with anti-fading mounting media containing DAPI. Results of three similar experiments show ABI-1 down-regulation interrupted lamellipodia formation. Images were acquired with a Zeiss LSM 510 confocal microscope. For details, and morphology of ABI-1 down-regulated MDA-MB-231 cells, see article by Wang and colleagues on p. 1031

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