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SKMEL/VEGF cells (1 x 107), mixed with 1 x 107 PT67 cells infected with retroviruses expressing DSCR1 isoform 4 specific SiRNA, were suspended in 0.5 ml of growth-factor reduced Matrigel and injected subcutaneously into Nu/Nu mouse. After 3 days, mouse was sacrificed. Tissues were harvested and photographed. For details, see article by Qin et al. on page 811.
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