On the Cover
HaCaT keratinocyte cells were transiently transfected with a plasmid encoding ZACD2, a variant of the candidate tumor suppressor protein ZAC. ZACD2 protein was detected using a rabbit anti-ZAC antibody followed by anti-rabbit Cy3 (red-orange). Involucrin was detected using a mouse anti-involucrin monoclonal antibody followed by anti-mouse FITC (green). Cells were counterstained with DAPI (blue). A ZACD2-negative cell on the left (blue nucleus) displays only basal involucrin staining. In contrast, the ZACD2-positive cell on the right (pink nucleus) shows abundant involucrin expression, indicating that ZACD2 expression leads to keratinocyte differentiation. For details, see the article by Basyuk et al. on page 483.