To refine 8p12 amplification and/or break alterations in breast cancers, sections from a series of primary tumor samples included in tissue microassays were hybridized with two fluorescent probes covering the UNC5D (FITC, green) and FGFR1 (TRITC, red) gene regions, respectively. In interphase nuclei (DAPI, blue) of this tumor section, the presence of clustered red and green signals (one dot each) was detected on the 8p12 wild-type allele, whereas the multiplication of red dots without green signal shows the amplification of the FGFR1 region (red) associated with a telomeric break of the 8p12 region leading to the loss of the upstream UNC5D region (green). For details, see the article by Gelsi-Boyer, et al. on page 655.

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