HBx-expressing ChangX-34 cells released from the double thymidine block were treated with 100 ng/ml of nocodazole for 12 hrs. Round mitotic cells were collected, washed, and seeded on poly-L-lysine coated cover glasses. The cells were then allowed to progress for a further 24 hrs and were fixed with a methanol/acetone (1:1) solution. Micronuclei formation was visualized after staining with DAPI, and the boundary of the cells was visualized by incubation with anti-PKC-α antibody and cyc3-conjugated anti-rabbit IgG antibody. For details, see article by Yun et al. on page 159.

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