Confocal immunofluorescence demonstrating colocalization of the MUC1 C-terminal subunit and nucleolin in cells of a primary breast carcinoma. The MUC1 C-terminal subunit was detected with a hamster antibody against the MUC1 cytoplasmic domain followed by a FITC-conjugated goat anti-hamster IgG(green). Nucleolin was detected with a mouse anti-nucleolin antibody and a secondary Texas Red-conjugated goat anti-mouse IgG (red). The merged image showing colocalization of the MUC1 C-terminal subunit and nucleolin (green+red=yellow) indicates targeting of the MUC1 C-terminal subunit to nucleoli. For details, see the article by Li et al. on page 765.

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