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Molecular Cancer Research

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Research Article

Characterization and Evidence of the miR-888 Cluster as a Novel Cancer Network in Prostate

Tsuyoshi Hasegawa, Garrison Glavich, Mary Pahuski, Aleena M Short, Oliver John Semmes, Lifang Yang, Vitold Galkin, Richard R. Drake and Aurora Esquela-Kerscher
Tsuyoshi Hasegawa
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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Garrison Glavich
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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Mary Pahuski
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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Aleena M Short
Eastern Virginia Medical School
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Oliver John Semmes
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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Lifang Yang
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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Vitold Galkin
Eastern Virginia Medical School
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Richard R. Drake
Department of Cell and Molecular Pharmacology and Experimental Therapeutics and MUSC Proteomics Center, Medical University of South Carolina
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Aurora Esquela-Kerscher
Microbiology and Molecular Cell Biology, Eastern Virginia Medical School
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  • For correspondence: kerschae@evms.edu
DOI: 10.1158/1541-7786.MCR-17-0321
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Abstract

Abstract: Prostate cancer afflicts 1 in 7 men and is the second leading cause of male cancer-related deaths in the United States. MicroRNAs (miRNAs), an extensive class of ~22 nucleotide non-coding RNAs, are often aberrantly expressed in tissues and fluids from prostate cancer patients but the mechanism of how specific miRNAs regulate prostate tumorigenesis and metastasis are poorly understood. Here, miR-888 was identified as a novel prostate factor that promotes proliferation and migration. miR-888 resides within a genomic cluster of 7 miRNA genes (mir-892c, mir-890, mir-888, mir-892a, mir-892b, mir-891b, mir-891a) on human chromosome Xq27.3. Moreover, as miR-888 also maps within HPCX1, a locus associated with susceptibility and/or hereditary prostate cancer, it was hypothesized that additional miRNA cluster members also play functional roles in the prostate. Expression analysis determined that cluster members were similarly elevated in metastatic PC3-ML prostate cells and their secreted exosomes, as well as enriched in expressed prostatic secretions (EPS) urine derived-exosomes obtained from clinical patients with high-grade prostate cancer. In vitro assays revealed that miR-888 cluster members selectively modulated PC3-derived and LNCaP cell proliferation, migration, invasion, and colony formation. Mouse xenograft studies verified miR-888 and miR-891a as pro-oncogenic factors that increased prostate tumor growth in vivo. Further analysis validated RBL1, KLF5, SMAD4 and TIMP2 as direct miR-888 targets and that TIMP2 is also co-regulated by miR-891a. This study provides the first comprehensive analysis of the entire miR-888 cluster and reveals biological insight. Implications: This work reveals a complex non-coding RNA network in the prostate that could be developed as effective diagnostic and therapeutic tools for advanced prostate cancer.

  • Received June 20, 2017.
  • Revision received November 10, 2017.
  • Accepted December 27, 2017.
  • Copyright ©2018, American Association for Cancer Research.

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Published OnlineFirst January 12, 2018
doi: 10.1158/1541-7786.MCR-17-0321

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Characterization and Evidence of the miR-888 Cluster as a Novel Cancer Network in Prostate
Tsuyoshi Hasegawa, Garrison Glavich, Mary Pahuski, Aleena M Short, Oliver John Semmes, Lifang Yang, Vitold Galkin, Richard R. Drake and Aurora Esquela-Kerscher
Mol Cancer Res January 12 2018 DOI: 10.1158/1541-7786.MCR-17-0321

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Characterization and Evidence of the miR-888 Cluster as a Novel Cancer Network in Prostate
Tsuyoshi Hasegawa, Garrison Glavich, Mary Pahuski, Aleena M Short, Oliver John Semmes, Lifang Yang, Vitold Galkin, Richard R. Drake and Aurora Esquela-Kerscher
Mol Cancer Res January 12 2018 DOI: 10.1158/1541-7786.MCR-17-0321
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Molecular Cancer Research
eISSN: 1557-3125
ISSN: 1541-7786

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