| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
1 Departments of Medical Oncology and 2 Biostatistics, Dana-Farber Cancer Institute, Boston, MA;
3 Department of Pathology, Brigham and Women's Hospital, Boston, MA;
4 Department of Pathology, Faulkner and Brigham and Women's Hospital, Boston, MA;
5 Department of Pathology, Beth-Israel Deaconess Medical Center, Boston, MA;
6 Harvard Medical School and 7 Harvard School of Public Health, Boston, MA;
8 Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD;
9 National Cancer Institute, Bethesda, MD; and
10 Department of Pathology, Duke University Medical Center, Durham, NC
Requests for reprints: Kornelia Polyak, Dana-Farber Cancer Institute, 44 Binney St. D740C, Boston, MA 02115. Phone: (617) 632-2106; Fax: (617) 632-4005. E-mail: Kornelia_Polyak{at}dfci.harvard.edu
Gene expression patterns in ductal carcinoma in situ (DCIS), and in invasive, and metastatic breast tumors were determined using serial analysis of gene expression (SAGE). We used mRNA in situ hybridization to examine gene expression at the cellular level and immunohistochemistry on tissue microarrays to determine association between gene expression patterns and histopathologic characteristics of the tumors. We found that that the most dramatic transcriptome change occurs at the normal to DCIS transition, while there is no clear universal "in situ" or "invasive" tumor molecular signature. From the 16,430 transcripts analyzed, we identified only 5 and 11 that were preferentially up-regulated in DCIS and invasive tumors, respectively. The majority of invasive cancer specific SAGE tags correspond to novel genes. The genes we identified may define biologically and clinically meaningful subgroups of DCIS with a high risk of progression to invasive disease.
This article has been cited by other articles:
|
|
 |
|
  S. Zhou, J. Si, T. Liu, and J. W. DeWille PIASy Represses CCAAT/Enhancer-binding Protein {delta} (C/EBP{delta}) Transcriptional Activity by Sequestering C/EBP{delta} to the Nuclear Periphery J. Biol. Chem., July 18, 2008; 283(29): 20137 - 20148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kijima, J. Ye, C. Glackin, and S. Chen CCAAT/Enhancer Binding Protein {delta} Up-regulates Aromatase Promoters I.3/II in Breast Cancer Epithelial Cells Cancer Res., June 1, 2008; 68(11): 4455 - 4464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vincent-Salomon, C. Lucchesi, N. Gruel, V. Raynal, G. Pierron, R. Goudefroye, F. Reyal, F. Radvanyi, R. Salmon, J.-P. Thiery, et al. Integrated Genomic and Transcriptomic Analysis of Ductal Carcinoma In situ of the Breast Clin. Cancer Res., April 1, 2008; 14(7): 1956 - 1965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Allred, Y. Wu, S. Mao, I. D. Nagtegaal, S. Lee, C. M. Perou, S. K. Mohsin, P. O'Connell, A. Tsimelzon, and D. Medina Ductal Carcinoma In situ and the Emergence of Diversity during Breast Cancer Evolution Clin. Cancer Res., January 15, 2008; 14(2): 370 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. Pearson and T. Hunter Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells J. Cell Biol., December 31, 2007; 179(7): 1555 - 1567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fabian Tamoxifen or Raloxifene in Postmenopausal Women for Prevention of Breast Cancer: A Tale of Two Choices Counterpoint Cancer Epidemiol. Biomarkers Prev., November 1, 2007; 16(11): 2210 - 2212. [Full Text] [PDF]
|
|
|
|
|
|
S. Esseghir, A. Kennedy, P. Seedhar, A. Nerurkar, R. Poulsom, J. S. Reis-Filho, and C. M. Isacke Identification of NTN4, TRA1, and STC2 as Prognostic Markers in Breast Cancer in a Screen for Signal Sequence Encoding Proteins Clin. Cancer Res., June 1, 2007; 13(11): 3164 - 3173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Hartwell, B. Muir, F. Reinhardt, A. E. Carpenter, D. C. Sgroi, and R. A. Weinberg The Spemann organizer gene, Goosecoid, promotes tumor metastasis PNAS, December 12, 2006; 103(50): 18969 - 18974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dolled-Filhart, L. Ryden, M. Cregger, K. Jirstrom, M. Harigopal, R. L. Camp, and D. L. Rimm Classification of breast cancer using genetic algorithms and tissue microarrays. Clin. Cancer Res., November 1, 2006; 12(21): 6459 - 6468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lupu and J. A. Menendez Targeting Fatty Acid Synthase in Breast and Endometrial Cancer: An Alternative to Selective Estrogen Receptor Modulators? Endocrinology, September 1, 2006; 147(9): 4056 - 4066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Okada, M. Kobayashi, H. Tanaka, T. Kobayashi, H. Tazawa, Y. Iuchi, K. Onuma, M. Hosokawa, M. C. Dinauer, and N. H. Hunt The Role of Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Derived Reactive Oxygen Species in the Acquisition of Metastatic Ability of Tumor Cells Am. J. Pathol., July 1, 2006; 169(1): 294 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Schuetz, M. Bonin, S. E. Clare, K. Nieselt, K. Sotlar, M. Walter, T. Fehm, E. Solomayer, O. Riess, D. Wallwiener, et al. Progression-Specific Genes Identified by Expression Profiling of Matched Ductal Carcinomas In situ and Invasive Breast Tumors, Combining Laser Capture Microdissection and Oligonucleotide Microarray Analysis. Cancer Res., May 15, 2006; 66(10): 5278 - 5286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yao, S. Weremowicz, B. Feng, R. C. Gentleman, J. R. Marks, R. Gelman, C. Brennan, and K. Polyak Combined cDNA array comparative genomic hybridization and serial analysis of gene expression analysis of breast tumor progression. Cancer Res., April 15, 2006; 66(8): 4065 - 4078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Krop, A. Marz, H. Carlsson, X. Li, N. Bloushtain-Qimron, M. Hu, R. Gelman, M. S. Sabel, S. Schnitt, S. Ramaswamy, et al. A Putative Role for Psoriasin in Breast Tumor Progression Cancer Res., December 15, 2005; 65(24): 11326 - 11334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thangaraju, M. Rudelius, B. Bierie, M. Raffeld, S. Sharan, L. Hennighausen, A-M. Huang, and E. Sterneck C/EBP{delta} is a crucial regulator of pro-apoptotic gene expression during mammary gland involution Development, November 1, 2005; 132(21): 4675 - 4685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Zucchi, E. Mento, V. A. Kuznetsov, M. Scotti, V. Valsecchi, B. Simionati, E. Vicinanza, G. Valle, S. Pilotti, R. Reinbold, et al. Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis PNAS, December 28, 2004; 101(52): 18147 - 18152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Alexander, A. L. Stegner, C. Wagner-Mann, G. C. Du Bois, S. Alexander, and E. R. Sauter Proteomic Analysis to Identify Breast Cancer Biomarkers in Nipple Aspirate Fluid Clin. Cancer Res., November 15, 2004; 10(22): 7500 - 7510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, H. Sun, J. Drake, F. Kittrell, M. C. Abba, L. Deng, S. Gaddis, A. Sahin, K. Baggerly, D. Medina, et al. From Mice to Humans: Identification of Commonly Deregulated Genes in Mammary Cancer via Comparative SAGE Studies Cancer Res., November 1, 2004; 64(21): 7748 - 7755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Namba, J. E. Maglione, L. J.T. Young, A. D. Borowsky, R. D. Cardiff, C. L. MacLeod, and J. P. Gregg Molecular Characterization of the Transition to Malignancy in a Genetically Engineered Mouse-Based Model of Ductal Carcinoma In situ Mol. Cancer Res., August 1, 2004; 2(8): 453 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Leonard and S. M. Swain Ductal Carcinoma In Situ, Complexities and Challenges J Natl Cancer Inst, June 16, 2004; 96(12): 906 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mehrotra, M. Vali, M. McVeigh, S. L. Kominsky, M. J. Fackler, J. Lahti-Domenici, K. Polyak, N. Sacchi, E. Garrett-Mayer, P. Argani, et al. Very High Frequency of Hypermethylated Genes in Breast Cancer Metastasis to the Bone, Brain, and Lung Clin. Cancer Res., May 1, 2004; 10(9): 3104 - 3109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Burstein, K. Polyak, J. S. Wong, S. C. Lester, and C. M. Kaelin Ductal Carcinoma in Situ of the Breast N. Engl. J. Med., April 1, 2004; 350(14): 1430 - 1441. [Full Text] [PDF]
|
|
|
|
 |
|
 H. T. Mouridsen and N. Brunner Clinical Infrastructures to Support Proteomic Studies of Tissue and Fluids in Breast Cancer Mol. Cell. Proteomics, April 1, 2004; 3(4): 302 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Morrow The Certainties and the Uncertainties of Ductal Carcinoma In Situ J Natl Cancer Inst, March 17, 2004; 96(6): 424 - 425. [Full Text] [PDF]
|
|
|
|
|
|
A. K. Meeker, J. L. Hicks, E. Gabrielson, W. M. Strauss, A. M. De Marzo, and P. Argani Telomere Shortening Occurs in Subsets of Normal Breast Epithelium as well as in Situ and Invasive Carcinoma Am. J. Pathol., March 1, 2004; 164(3): 925 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Fuja, F. Lin, K. E. Osann, and P. J. Bryant Somatic Mutations and Altered Expression of the Candidate Tumor Suppressors CSNK1{epsilon}, DLG1, and EDD/hHYD in Mammary Ductal Carcinoma Cancer Res., February 1, 2004; 64(3): 942 - 951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Porter, S. Weremowicz, K. Chin, P. Seth, A. Keshaviah, J. Lahti-Domenici, Y. K. Bae, C. L. Monitto, A. Merlos-Suarez, J. Chan, et al. A neural survival factor is a candidate oncogene in breast cancer PNAS, September 16, 2003; 100(19): 10931 - 10936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Celis, P. Gromov, I. Gromova, J. M. A. Moreira, T. Cabezon, N. Ambartsumian, M. Grigorian, E. Lukanidin, P. thor Straten, P. Guldberg, et al. Integrating Proteomic and Functional Genomic Technologies in Discovery-driven Translational Breast Cancer Research Mol. Cell. Proteomics, June 1, 2003; 2(6): 369 - 377. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
